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藜麦籽粒酸性氨基酸组分遗传特性分析与评价
引用本文:王倩朝,刘永江,李莉,李淑贤,孔治有,刘俊娜,环秀菊,覃鹏. 藜麦籽粒酸性氨基酸组分遗传特性分析与评价[J]. 中国粮油学报, 2020, 35(8): 62
作者姓名:王倩朝  刘永江  李莉  李淑贤  孔治有  刘俊娜  环秀菊  覃鹏
作者单位:云南农业大学农学与生物技术学院
基金项目:云南省院士工作站(2019IC006)
摘    要:藜麦具有全营养特点及重要利用价值,为揭示藜麦氨基酸组分遗传特性和评价藜麦种质资源品质状况,本研究以筛选出的89个藜麦高代品系籽粒为材料进行酸水解处理并以氨基酸自动分析仪测定其酸性氨基酸组分及其含量,结果表明:17种酸性氨基酸平均变异系数为22.97%,表明在不同藜麦品系之间存在较大的遗传特性差异,其中Glu的变异系数为14.4%、相对变幅较小,GlyCysTyrPheHisPro6种氨基酸的变异系数均高于20%,尤其是Met的变异系数高达77.1%、遗传变异最大相关分析表明绝大部分氨基酸组分间存在极显著正相关关系,主成分分析显示5主成分对变异的累计贡献率高达92.55%,聚类分析将89个藜麦品系分为5类、其遗传特性与来源和颜色没有必然联系经分析筛选出滇藜-59、滇藜-71、滇藜-72、滇藜-73、滇藜-78和滇藜-926个具有较高的综合利用价值的高代品系。本研究可为藜麦氨基酸组分遗传特性分析与评价及新品种选育提供了理论参考和材料基础。

关 键 词:藜麦  酸性氨基酸组分 [sup_s]遗传特性[sup_e] [sup_s]相关性分析 [sup_e]主成分分析  聚类[sup_s]分析[sup_e]
收稿时间:2019-11-09
修稿时间:2020-01-20

Analysis and Evaluation of Genetic Characteristics of Acidic Amino Acids in Quinoa Seeds
Abstract:Quinoa has full nutritional characteristics and important utilization value. For revealing the genetic characteristics of quinoa germplasm resources and evaluating the genetic status of quinoa germplasm resources, the seeds of 89 high-generation lines of quinoa were used for determining of acidic amino acid components and contents by acid hydrolysis treatment using an automatic amino acid analyzer in this study, The results showed that the average coefficient of variation of 17 acidic amino acids was 22.97%, indicating that there are large differences in genetic characteristics between different quinoa lines, among which Glu is relatively stable with 14.4% coefficient of variation, The coefficient of variation of six amino acids such as Gly ,Cys, Tyr, Phe, His and Pro was higher than 20%, especially that of Met was as high as 77.1%, which showed the maximum genetic variation; correlation analysis showed that there was a very significant positive correlation among most amino acid components; principal component analysis showed that the cumulative contribution rate of the five principal components to the variation was as high as 92.55%; 89 quinoa lines were divided into five categories in cluster analysis, and their genetic characteristics were not necessarily related to the sources and seed colors; the six lines such as Dianli-59, Dianli -71, Dianli -72, Dianli -73, Dianli -78 and Dianli-92 were selected for their high comprehensive utilization value. This study could provide an important theoretical and material basis for the analysis and evaluation of the genetic characteristics of quinoa amino acid components and the selection of new varieties.
Keywords:quinoa   acidic amino acid components   genetic characteristics   correlation analysis   principal component analysis   cluster analysis
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