马尾藻多糖的提取及其免疫活性

目的:研究马尾藻多糖(SP)的提取纯化和免疫活性。方法:采用超声波破碎法从马尾藻中提取制备马尾藻多糖(SP),破壁时间15min,破壁功率为108W,80℃水浴8h,过滤,离心,浓缩,用酒精沉淀出粗多糖,40℃真空干燥,Sevage法除蛋白,DE-52纯化除色素,透析,得到马尾藻细多糖(SP)并进行免疫实验。结果:多糖剂量20、40、60mg/kg·d时对小鼠脾的重量显著增加,p<0.05;当多糖剂量为40、60mg/kg·d时对小鼠腋下淋巴结的重量显著增加,p<0.05,对单核巨噬细胞的吞噬功能加强有显著影响,能明显提高小鼠碳粒廓清速率,提高吞噬指数α,p<0.05,并对巨噬细胞增生以及淋巴小结形成有明显作用;当多糖剂量为60mg/kg·d时对肝增重也达到显著水平,p<0.05。结论:马尾藻多糖有免疫促进作用。

Studies on Extraction of Polysaccharides from Sargassum and Its Immunocompetence

Purpose: To study extraction, purification and immunocompetence of Sargassum polysaccharides (sp). Methods: The SP was extracted with pretreating the Sargassum with ultrasonication for 15 minutes, then extracted 8h at 80℃, filtrated, certrifuged, condensed, and depositing the sulphated polysaccharides with alohol, and dried. Then the protein was eliminated by sevage, and the SP was purified by DEAE-cellulosecolume, dialysed, dried and doing the immunocompetence experiment. Results: The SP can increase the weight of the spleen of mice significantly (p<0.05), when the dose of polysaccharides reached respectively 20, 40 and 60mg/kg·d. It can increase the weight of the lymphonodi axillares significantly, multiplicate macrophage, quicken the cleanup speed of the lymphond nodule, strengthen the phagocytosis of monocytle-macrophages and increase the index of phagocytcis significantly(p<0.05). When the dose of polysaccharides is 40 and 60mg/kg·d, it will increase the weight of the liver of mise significantly, when the dose of polysaccharides is 60mg/kg·d.Conclusion: The SP can improve the immunocompetence.