Antioxidant activity of evening primrose phenolics in sunflower and rapeseed oils |
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Authors: | tefan Schmidt Ivana Niklov Jan Pokorný Pavel Farka Stanislav Sekretr |
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Affiliation: | Štefan Schmidt,Ivana Niklová,Jan Pokorný,Pavel Farkaš,Stanislav Sekretár |
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Abstract: | Crude ethanol/ethyl acetate extracts of industrial evening primrose (Oenothera biennis L.) seed meal were separated into six fractions using the Sephadex LH‐20 column chromatography and 96% aqueous ethanol as a mobile phase. Their antioxidant activities were tested in sunflower and rapeseed oils by using an Oxidograph apparatus at a temperature of 110 °C. Only the fractions III and IV displayed a pronounced antioxidant activity while the other fractions were either inactive or even pro‐oxidative. The active fractions contained phenolic acids and their esters; gallic acid, methyl and ethyl gallates, protocatechuic acid and its methyl ester were identified by GC/MS. Catechin was present, too, but exhibited only moderate antioxidant activity in sunflower oil. |
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Keywords: | antioxidants evening primrose seed Oenothera biennis phenolics rapeseed oil stability against oxidation sunflower oil |
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