| 溶菌酶在硅烷化石英表面吸附的压电传感监测 |
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| 引用本文： | 康琪,李敏,马小隆,李文鹏,申大忠.溶菌酶在硅烷化石英表面吸附的压电传感监测[J].传感器与微系统,2001,20(12):8-9,12. |
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| 作者姓名： | 康琪 李敏 马小隆 李文鹏 申大忠 |
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| 作者单位： | 1. 山东科技大学化工系,
2. 山东大学化学与化工学院, |
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| 基金项目： | 山东省自然科学基金;Q99B01; |
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| 摘 要： | 石英表面经硅烷修饰后 ,可通过憎水性相互作用吸附溶菌酶 ,该吸附动力学过程以压电传感器实时监测。结果表明溶菌酶以单分子层吸附 ,吸附仅部分可逆 ,吸附和解吸的速率常数分别为ka =36 .4mol-1.kg .s-1,kd =8.92 × 10 -3 s-1,且可逆吸附比及饱和单层的吸附密度随溶菌酶浓度增加而增加
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| 关 键 词： | 吸附 溶菌酶 液隔电极式压电传感器 |
| 文章编号： | 1000-9787(2001)12-0008-02 |
Monitor the adsorption of lysozyme on methylsilaned quartz surface by a piezoelectric sensor |
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| KANG Qi ,LI Min ,MA Xiao long ,LI Wen peng ,SHEN Da zhong.Monitor the adsorption of lysozyme on methylsilaned quartz surface by a piezoelectric sensor[J].Transducer and Microsystem Technology,2001,20(12):8-9,12. |
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| Authors: | KANG Qi LI Min MA Xiao long LI Wen peng SHEN Da zhong |
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| Affiliation: | KANG Qi 1,LI Min 1,MA Xiao long 1,LI Wen peng 2,SHEN Da zhong 2 |
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| Abstract: | Quartz surface that was modified by dichchlorodimethylsilane can adsorb lysozyme by the hydrophobic interaction. This adsorption kinetics process was monitored real time by a piezoelectric sensor. It was shown that lysozyme was adsorbed in monolayer and partly reversible. The rate constants for adsorption and desorption were estimated to be k a=36.4mol -1 .kg.s -1 and k d=8.92×10 -3 s -1 ,respectively.The ratio for reversible adsorption and the adsorption densities of saturation monolayer increases with increasing lysozyme concentration. |
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| Keywords: | adsorption lysozyme electrode separated piezoelectric sensor |
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