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分光光度法测定鸡肉中的羟脯氨酸含量
引用本文:罗凤莲,夏延斌,欧阳建勋,王燕,夏晓凤. 分光光度法测定鸡肉中的羟脯氨酸含量[J]. 食品科技, 2010, 0(1)
作者姓名:罗凤莲  夏延斌  欧阳建勋  王燕  夏晓凤
作者单位:湖南农业大学食品科学技术学院;
基金项目:湖南省2008年标准化战略项目
摘    要:采用分光光度法测定鸡肉中的羟脯氨酸(Hyp)含量。结果表明:鸡肉中的羟脯氨酸的含量以农家饲养的土鸡含量为最高,达0.22%,其次为市场购买后再于农家饲养的鸡肉,达0.15%;对于同一鸡肉样品,鸡腿肉中的羟脯氨酸含量明显高于鸡胸肉中羟脯氨酸含量。该方法测定的标准偏差为0.00443,变异系数为3.50%,平均回收率分别为89.5%。

关 键 词:鸡肉  羟脯氨酸  分光光度法  胶原蛋白  

Determination of hydroxyproline in chicken by spectrophotometer
LUO Feng-lian,XIA Yan-bin,OUYANG Jian-xun,WANG Yan,XIA Xiao-feng. Determination of hydroxyproline in chicken by spectrophotometer[J]. Food Science and Technology, 2010, 0(1)
Authors:LUO Feng-lian  XIA Yan-bin  OUYANG Jian-xun  WANG Yan  XIA Xiao-feng
Affiliation:LUO Feng-lian,XIA Yan-bin,OUYANG Jian-xun,WANG Yan,XIA Xiao-feng (College of Food Science , Technology,Hunan Agricultural University,Changsha 410128)
Abstract:The hydroxyproline content in chicken was determined by spectrophotometer in this study. The results showed that:The highest hydroxyproline content was 0.22% in chicken which was feed in village peasant family,next was 0.15% in chicken which was first bought in market and then feed in village peasant family. The hydroxyproline content in chickens leg was much higher than in chickens chest. The standard deviation of this method was 0.00443,coefficient of variation was 3.50%,average returns-ratio was 89.5%.
Keywords:chicken  hydroxyproline  spectrophotometer  collogen  
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