海洋细菌B1109产胞外多糖的培养条件

生物多糖是在食品、化工与医药领域有着广泛用途的一类高分子生物物质。为了考察海洋细菌B1109产胞外多糖培养的条件,对从海泥中分离到的1株海洋细菌B1109产胞外多糖的碳源、氮源、碳氮浓度等营养元素和培养基初始pH值、培养基装量、接种量、培养温度等培养条件进行了研究。该菌株产胞外多糖最佳碳源为葡萄糖,最佳氮源为硫酸铵,质量浓度分别为20和4 g/L;最佳培养条件为:培养基初始pH 8.0,培养基装量400 mL/L,接种量6%,培养温度24℃。在此条件下培养120 h,海洋细菌B1109产胞外多糖4.29 g/L。

Exopolysacchrides production by marine bacterium B1109

Exopolysaccharides-producing strain B1109 was isolated from oozed sea water and the culture conditions were investigated.The optimal culture conditions for exopolysaccharides production were as follows: 20 g/L glucose,4 g/L ammonium sulfate,the initial pH 8.0,the loading sample 400 mL/L,the inoculum 6% and the temperature 24 ℃.When cultured under such conditions for 120 h,the strain B1109 could produce 4.29 g/L exopolysaccharids.