Proteolysis of ultra-high pressure homogenised treated milk during refrigerated storage |
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Authors: | J Pereda V FerragutM Buffa B GuamisAJ Trujillo |
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Affiliation: | Centre Especial de Recerca Planta de Tecnologia dels Aliments (CERPTA), XaRTA, XiT, Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain |
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Abstract: | Plasmin residual activity and its relation to proteolysis of milk subjected to ultra-high pressure homogenisation (UHPH; 200–300 MPa, inlet temperature = 30 °C and 40 °C) and to a high-pasteurisation treatment (90 °C, 15 s) were studied during refrigerated storage. Proteolysis was examined by capillary electrophoresis, HPLC peptide profiles, pH 4.6-soluble nitrogen and free amino acids. Inactivation of plasmin increased as homogenisation pressure did. Extensive proteolysis, was observed in 200 MPa 40 °C milk, due to its higher native and microbial enzyme contents, compared with the other samples. In general, hydrolysis of β-casein, hydrophobic peptide and pH 4.6-soluble nitrogen levels increased with higher residual plasmin activity, while hydrophilic peptides were not affected by the different treatments applied. β-Lactoglobulin was denatured to a greater extent by thermal treatment than by UHPH treatments. This study provides further insight into how UHPH treatments influence milk properties. |
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Keywords: | Ultra-high pressure homogenisation Proteolysis Milk proteins |
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