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The trypsin inhibitors present in seed of different grain legume species and cultivar
Authors:Eva Guillamón  Mercedes M Pedrosa  Carmen Burbano  Carmen Cuadrado  María de Cortes Sánchez  Mercedes Muzquiz
Affiliation:1. Dpto Tecnología de Alimentos, SGIT-INIA, Apdo 8111, 28080 Madrid, Spain;2. Dpto Nutrición y Bromatología II, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain
Abstract:Trypsin inhibitors in a selection of grain legume seeds from different species and cultivars were studied. The results showed that trypsin inhibition content ranged from negligible in Lupinus spp. to very high in Glycine max. Although there is variation among cultivars, generally the highest TIU mg−1 sample values occured in soybean (43–84) and common bean (21–25). Inhibitor content of different Lathyrus cultivars, ranged from 19–30 TIU mg−1 sample. This was higher than the contents in chickpea (15–19 TIU mg−1 sample) and pea (6–15 TIU mg−1 sample). Lentil and faba bean had low values in most cvs (3–8, and 5–10 TIU mg−1 sample, respectively). Trypsin inhibitor isoform analyses showed that the amount of TI detected, varied with legume species and variety.
Keywords:Legume seeds  Trypsin inhibitors  Zymogram gel
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