Analysis of mycotoxin fumonisins in corn products by high-performance liquid chromatography coupled with evaporative light scattering detection |
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Authors: | Jiansheng Wang Ying ZhouQiaomei Wang |
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Affiliation: | Department of Horticulture, Zhejiang University, The State Agriculture Ministry Laboratory of Horticultural, Plant Growth Development and Biotechnology, Hangzhou 310029, China |
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Abstract: | Fumonisins are mycotoxins produced by the fungus Fusarium verticillioides, which is a widespread pathogen of corn. The mycotoxins are known to cause fatal diseases in some domestic animals and have been linked to human esophageal cancer in China and South Africa. Here, we describe a simple method for direct and quantitative analysis of the toxins in food products. The method is based on high-performance liquid chromatography coupled with an evaporative laser scattering detector (HPLC–ELSD) without any prior derivatization of the samples. Using this method, we have analyzed corn-based food samples from central markets in eastern China. The results showed that FB1 was the main contaminant in the samples. The overall level of fumonisin contamination was relatively low, with a range of 0.25–1.80 μg/g (mean 0.74 μg/g) in 66.7% (16 of 24) of corn samples from Middle-eastern Area, 0.21–0.29 μg/g (mean 0.24 μg/g) in 28.6% (6 of 21) of corn samples from Northeastern Area, and 0.30–3.13 μg/g (mean 0.47 μg/g) in 30.0% (6 of 20) of corn samples from Southeastern Area. |
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Keywords: | Fumonisin Fusarium verticillioides HPLC&ndash ELSD Corn |
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