Influence of salt and pH on the solubility and structural characteristics of transglutaminase-treated wheat gluten hydrolysate |
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Authors: | Kingsley K. Agyare Youling L. Xiong Kwaku Addo |
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Affiliation: | 1. Department of Animal and Food Sciences, 206 Garrigus Building, University of Kentucky, Lexington, KY 40546, USA;2. Department of Nutrition and Food Science, University of Kentucky, Lexington, KY 40506, USA |
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Abstract: | Hydrolyzed wheat gluten (GH, 77–85% protein) was prepared by limited chymotrypsin digestion at 37 °C for 4 h (degree of hydrolysis = 6.4%) and 15 h (degree of hydrolysis = 10.3%). Microbial transglutaminase (MTGase) treatment (55 °C for 1 h, or 5 °C for 18 h) effect on the solubility and structural characteristics of GH was examined under selected food processing conditions (pH 4.0–7.0, 0–0.6 M NaCl). The MTGase treatment increased solubility of GH by 3–29-fold (P < 0.05) within pH 4.0–7.0. Addition of 0.6 M NaCl or changing the conditions of MTGase incubation did not significantly alter solubility characteristics of GH. The MTGase treatment decreased surface hydrophobicity, and increased carboxyl groups in GH, suggesting cross-linking and deamidation. Fluorescence and UV spectra attributed the improved GH solubility to MTGase-induced polar environment, and partial masking of some nonpolar aromatic amino acids possibly due to high-molecular-weight polypeptides formed. |
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Keywords: | Hydrolyzed wheat gluten Structural properties Solubility Microbial transglutaminase |
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