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Fatty acid composition of seed oil of different Sorghum bicolor varieties
Authors:Sajid Mehmood  Ilkay Orhan  Zaheer Ahsan  Sinem Aslan  Muhammad Gulfraz
Affiliation:1. Department of Biochemistry, University of Arid Agriculture Rawalpindi, Pakistan;2. Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey;3. Oil Seed Program, National Agriculture Research Center (NARC), Islamabad, Pakistan;4. Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06100 Ankara, Turkey
Abstract:In order to find out new sources of premium quality edible oil in the country, seeds of ten varieties of Sorghum bicolor were initially analyzed for their total oil contents. The seed oil was later fractionated into eight fatty acids including two new saturated fatty acids. The oil contents were determined by Soxhlet method and compared with the results obtained by NMR analysis. The total oil contents in the seeds of sorghum ranged from 5.0 to 8.2 % (w/w), indicating non significant difference obtained by two different techniques. The results revealed that oleic acid (31.12–48.99%), Palmitoleic acid (0.43–0.56%), linoleic acids (27.59–50.73%), linolenic acid (1.71–3.89%), stearic acid (1.09–2.59%) and palmitic acid (11.73–20.18%) was present in the seed oil of different sorghum varieties when analyzed by GC–MS. It was observed that in most of the varieties polyunsaturated fatty acids (PUFA) were higher than monounsaturated fatty acids (MUFA). The two atypical SFAs, octanedioic (C8:0) and azelaic acid (C9:0) were found in some varieties. These results suggest that these S. bicolor varieties could be additional sources of edible oil due to presence of clinically important saturated and high concentration of unsaturated fatty acids. A large scale production of the seed oil after refining process can contribute towards alleviation of edible oil shortage in the country with increased use of premium quality oil.
Keywords:Sorghum bicolor   Fatty acid   Gas chromatography (GC)   Nuclear magnetic resonance (NMR)
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