| 液相色谱-串联质谱分离鉴定树莓叶中黄酮类化合物 |
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| 引用本文： | 叶晓珂,秦沛,李伟,郭璐,唐伟伟,王硕.液相色谱-串联质谱分离鉴定树莓叶中黄酮类化合物[J].质谱学报,2011,32(5):271-277. |
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| 作者姓名： | 叶晓珂 秦沛 李伟 郭璐 唐伟伟 王硕 |
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| 作者单位： | 天津科技大学食品工程与生物技术学院，食品营养与安全省部共建教育部重点实验室，天津300457 |
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| 摘 要： | 树莓叶作为一种新的黄酮资源，为了分析鉴定其黄酮类化合物的结构，采用高效液相色谱-二极管阵列-离子阱多级质谱(HPLC-PDA-MSn)联用技术分离鉴定树莓叶乙醇提取物中的黄酮类化合物。通过离子阱多级质谱提供的准分子离子峰和多级碎片离子信息，分析得到了黄酮类化合物的相对分子质量、黄酮糖苷的组成结构、黄酮糖苷中糖的类型和多糖链的连接次序等。结合液相色谱相对保留时间、标准品和相关文献对照，对树莓叶乙醇提取物中的15个黄酮类化合物的可能结构进行推断。结果表明，树莓叶中有8个槲皮素衍生化的黄酮化合物和7个山奈酚衍生化的黄酮化合物，这与树莓中主要含有槲皮素和山奈酚衍生化的黄酮化合物报道一致。
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| 关 键 词： | 树莓叶 黄酮类化合物 分离鉴定 液相色谱-质谱法(HPLC-MSn) 离子阱 |
Separation and Identification of Flavonids of Leaves of Rubus idaeus by High Performance Liquid Chromatography Electrospray Ionization Multi-Stage Tandem Mass Spectrometry |
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| YE Xiao-ke,QIN Pei,LI Wei,GUO Lu,TANG Wei-wei,WANG Shuo.Separation and Identification of Flavonids of Leaves of Rubus idaeus by High Performance Liquid Chromatography Electrospray Ionization Multi-Stage Tandem Mass Spectrometry[J].Journal of Chinese Mass Spectrometry Society,2011,32(5):271-277. |
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| Authors: | YE Xiao-ke QIN Pei LI Wei GUO Lu TANG Wei-wei WANG Shuo |
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| Affiliation: | Key Laboratory of Food Nutrition and Safety, Ministry of Education,Tianjin University of Science and Technology, Tianjin 300457 |
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| Abstract: | To analysis and determinate the construction of flavonids in the leaves of raspberry as a new flavonids resource,the utility of high performance liquid chromatograph in combined with photodiode array detection and mass spectrometer technology(HPLC-PDA-MSn) was successfully applied to simultaneous separate and determinate 15 flavonoides in ethanol extracts of leaves of Rubus idaeus.It was analyzed that constructions of flavonoid aglycones,the types of glycogen,sequences of polysaccharide using the informatio... |
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| Keywords: | leaves of Rubus idaeus flavonoids separation and determination HPLC-MSn ion trap |
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