SARS病毒刺突蛋白片段在大肠杆菌中的表达和纯化

目的获得SARS病毒表面刺突蛋白片段。方法用PCR方法克隆SARSS2片段基因,并在大肠杆菌中进行表达。表达产物经SDSPAGE及Westernblot鉴定,阴离子交换层析纯化。结果SARS病毒S2片段基因在大肠杆菌中获得了高表达,表达量占总蛋白的50%以上;得到纯度大于90%的SARS蛋白样品。结论获得了能够应用于SARS疫苗研究的SARS蛋白样品。

Expression of Spike Protein Fragment of SARS Virus in E. coli and Purification of Expressed Product

Objective To obtain the spike protein fragment of SARS virus and use for the development of SARS vaccine.Methods The gene encoding S2 fragment of SARS virus was amplified by PCR and expressed in E.coli.The expressed product was identified by SDS-PAGE and Western blot and purified by DEAE-Sepharose FF ion exchange chromatography.Results The gene encoding S2 fragment of SARS virus was highly expressed in E.coli.The expressed product contained more than 50% of total somatic protein and reached a purity of more than 90% after purification.Conclusion The SARS virus protein for developing vaccine was obtained.