Zn2+对球等鞭金藻3011细胞膜电位和膜通透性的影响

目的:用亲脂性阴离子荧光染料双(1,3-二巴比妥酸)-三次甲基氧烯洛尔和碘化丙啶标记球等鞭金藻3011(Isochrysis galbana 3011),研究Isochrysis galbana 3011受Zn2+作用时膜电位和膜通透性的变化。方法:对处理藻液进行流式细胞技术检测和荧光显微镜镜检,通过对比实验设计,实验数据使用SPSS17.0统计软件进行方差分析。结果:5μg/L Zn2+作用4min可使Isochrysis galbana 3011细胞内荧光强度明显增强,并促使膜的通透性发生剧烈变化,与对照组比较具有统计学意义。结论:找到一种快速动态测定Isochrysis galbana 3011细胞膜状态的方法,发现5μg/L的Zn2+能引起Isochrysis galbana 3011细胞膜的部分去极化,并改变膜的通透性,Zn2+的吸收有可能与钠通道联动有关。

Effect of Zn2+ on Membrane and Membrane Permeability of Isochrysis galbana 3011

Flow cytometry and fluorescence microscope were used to explore the effect of Zn2+on membrane potential and membance permeability in Isochrysis galbana 3011.The processed microalga were detected by flow cytometry and fluorescence microscope with DiBAC4(3) and PI staining through single-factor tests.The statistical software of SPSS 17.0 was used for variance analysis of the data.The results showed that the membrane potential in macrophage was increased significantly and the membance permeability was changed after stimulated with Zn2+in 5μg/L for 4 min.Therefore,a dynamic membrane status was observed and 5 μ g/L Zn2+could lead to partial membrane depolarization and change the membance permeability of Isochrysis galbana 3011,which may be linked to sodium ion channel.