交联壳聚糖薄膜及其水凝胶骨架片的制备与控释性能探讨

针对活性物质生物利用度提高这一热点问题,本文以三聚磷酸钠为交联剂,研究了交联壳聚糖薄膜的相关结构性能,探讨了交联壳聚糖水凝胶骨架片对活性物质的控释性能。壳聚糖来源广泛、生物相容性好,FT-IR-ATR分析显示,通过交联反应壳聚糖薄膜上引入一定基团,增强了薄膜的三维网络结构,从而使其具有一定的溶胀吸水性能。同时,交联后的壳聚糖薄膜耐酸性能明显提升,通过扫描电镜观察,交联壳聚糖薄膜经模拟胃液运转后表面仍然致密,无破损,具有作为活性物质载体材料的潜质。另外,以5-ASA为模型活性物质,通过压制得到交联壳聚糖水凝胶骨架片,在模拟释放实验中,5-ASA在胃液中0.5~1 h的释放率明显降低。研究结果显示,交联改性壳聚糖在活性物质传递及药物控释等领域都具有良好的应用前景。

Preparation and Study of the Controlled Release Properties of Crosslinked Chitosan Film and its Hydrogel Matrix Tablet

The bioavailability enhancement of bioactive compounds is an interesting research topic. Sodium tripolyphosphate (STPP) was used as a cross-linking agent to study the structural properties of crosslinked chitosan film and the controlled release property of a crosslinked chitosan-based hydrogel matrix tablet. Chitosan has an abundant source and good biocompatibility. Fourier transform infrared spectroscopy-attenuated total reflectance (FT-IR-ATR) analysis showed that some chemical groups were introduced to chitosan film via crosslinking reactions, which improved the strength of the three-dimensional network structure, and endowed the film with certain swelling and water absorption properties. Meanwhile, the acid resistance of the chitosan film was significantly improved after crosslinking; scanning electron microscopy showed that after transport in simulated gastric fluid (SGF), the surface of the crosslinked chitosan film was still compact with no damage, and it had the potential to be a carrier material for bioactive substances. Moreover, 5-aminosalicylic acid (5-ASA) was used as a model bioactive compound, and a crosslinked chitosan-based hydrogel matrix tablet was prepared by compression. In the simulated release experiment, the release rate of 5-ASA in SGF was dramatically reduced. These results suggest that chitosan crosslinked with STPP could have a wide range of applications in the transport of bioactive substances and the controlled release of drugs.