| 杜仲疏松愈伤组织诱导条件的优化筛选 |
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| 引用本文： | 邱晓芳,朱笃,张志斌,段小华,王曼莹,游海.杜仲疏松愈伤组织诱导条件的优化筛选[J].食品科学,2006,27(11):375-378. |
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| 作者姓名： | 邱晓芳 朱笃 张志斌 段小华 王曼莹 游海 |
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| 作者单位： | 江西师范大学生命科学学院,江西省亚热带植物资源保护与利用重点实验室,江西,南昌,330022 |
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| 基金项目： | 教育部科学技术研究项目 |
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| 摘 要： | 杜仲愈伤组织生长过于致密从而导致难以实现悬浮培养,因此获得疏松、生长快速愈伤组织是实现杜仲细胞悬浮培养的前提.本文研究了不同外植体、激素和培养基对杜仲愈伤组织诱导率、疏松程度以及褐化率的影响,结果表明：上胚轴和子叶更容易被诱导为疏松愈伤组织,MS培养基是最适诱导培养基;适当增加6-BA浓度有助于褐化的减轻且提高愈伤组织疏松程度,杜仲疏松愈伤组织诱导最适激素配比为1.0mg/L 2,4-D＋1.0mg/L NAA＋1.0mg/L 6-BA.
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| 关 键 词： | 杜仲 疏松愈伤组织 诱导 |
| 文章编号： | 1002-6630（2006）11-0375-04 |
| 收稿时间： | 2006-08-20 |
| 修稿时间： | 2006-08-20 |
Optimization of Induction Conditions of Loose Callus from Eucommia Ulmoidues |
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| QIU Xiao-fang,ZHU Du,ZHAN Zhi-bing,DUAN Xiao-hua,WANG Man-ying,YOU Hai.Optimization of Induction Conditions of Loose Callus from Eucommia Ulmoidues[J].Food Science,2006,27(11):375-378. |
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| Authors: | QIU Xiao-fang ZHU Du ZHAN Zhi-bing DUAN Xiao-hua WANG Man-ying YOU Hai |
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| Affiliation: | Jiangxi Provincial Key Laboratory of Protection and Utilization of Subtopic Plant Resources, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China |
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| Abstract: | Callus from Eucommia ulmoides grows too rigidly to suspension culture,so rapidly vegetal and looses callus is the basis of it's cell suspension culture. The sterile seedling as explants,the effects of different explants,concentration of NAA,2,4-D and 6-BA and 3 basic medium on Eucommia ulmoides callus induction and growth were investigated. The results showed that the epicotyl and cotyledon are much easier and the MS medium is the best medium for callus induction. The results of L9(34) orthogonal test indicated that properly increasing 6-BA content could reduce darkening and improve loose callus formation,and the optimum hormone combination was 1.0mg/L2,4-D 1.0mg/LNAA 1.0mg/6-BA. |
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| Keywords: | Eucommia ulmoides loose callus induction |
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