高效液相色谱- 串联质谱法测定猪肉中10 种类固醇类激素残留

建立猪肉中类固醇类激素残留高效液相色谱- 串联质谱(LC-MS/MS)分析方法。样品经甲醇提取，固相萃取柱净化后采用LC-MS/MS 进行检测分析；并对样品前处理条件和质谱参数进行研究。结果表明：这些激素物质的线性范围均为0.5～100μg/kg；低、中、高3 种加标水平的加标回收率为71%～89%；最低检出限为0.2～2μg/kg。本方法快速简便、灵敏度高、选择性好、测定结果令人满意，可在各食品检测机构推广应用。

High-performance Liquid Chromatography Coupled with Tandem Mass Spectrometric Determination of Steroid Hormone Multi-Residues in Pork

A method using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) was proposed for the determination of steroid hormone multi-residues in pork. Analytes were extracted from samples with methanol and cleaned up using a solid-phase extraction cartridge prior to HPLC-MS/MS analysis. The proposed method exhibited a good linearity for 10 steroid hormones over the range of 0.5－100μg/kg and a limit of detection ranging from 0.2 to 2μg/kg. Mean recoveries for 10 steroid hormones in blank pork samples spiked at low, medium and high concentrations were between 71% and 88%, with relative standard deviations below 10%. The method is characteristic of rapidity, simplicity, good selectivity and high sensitivity, and therefore has a great popularization potential.