藿香与青蒿挥发油及其复合物抑菌活性及化学成分研究

采用实验分析与气相色谱-质谱(GC-MS)法,分别测定藿香、青蒿挥发油及其复合物的抑菌圈直径与挥发油成分,并利用化学计量学解析法对重叠的色谱峰进行解析,得到各组分的纯色谱曲线和质谱,再通过质谱库对解析的纯组分进行定性,用总体积积分法进行定量。结果表明,藿香与青蒿挥发油复合后综合抑菌能力增强,产生了新的活性化合物。藿香与青蒿挥发油复合物在质与量上与单味药相比均发生显著变化。藿香、青蒿挥发油及其复合物定性鉴定的组分分别为48、69和73个,分别占总含量的93.23%、88.85%和89.25%,新增1,1-二甲基-1,2,3,4-四氢化异喹啉和表蓝桉醇两个组分。复合物主要化学组分基本为两个单味药物的加和,单味药物组分的相对含量都发生了变化,其中藿香组分在复合物中的相对含量较高。藿香与青蒿挥发油复合物具有高效天然抑菌剂的开发价值。

Antibacterial Activity and Chemical Composition of Volatile Oils from Herba Agastaches Rugosae and Herba Artemisiae Annuae and Their Half-and-half Mixture

The chemical composition of volatile oils from Herba Agastaches Rugosae and Herba Artemisiae Annuae and their half-and-half mixture were determined by gas chromatography-mass spectrometry(GC-MS).The qualitative and quantitative analyses were conducted by chemometric resolution method and overall volume integration method,respectively.Meanwhile,both volatile oils and their half-and-half mixture were tested for antibacterial activity.Results indicated that half-and-half mixture of the above oils exhibited enh...