Function of cryopreserved human effector cells in antibody-dependent, cell-mediated cytotoxicity |
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Authors: | MA Bean RA Miller Y Kodera HW Herr |
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Abstract: | Cells active in an ADCC model were studied for their ability to withstand cryopreservation. For this model, we used the [3H]proline microcytotoxicity assay with fibroblast target cells and a serum containing lymphocyte-dependent antibody reactive with the alloantigens on those target cells. Human blood effector cells active in this system can be successfully cryopreserved with minor dimunition in function with standard techniques and concentrations between 9 and 12% DMSO. |
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