Immobilized penicillin G acylase on mesoporous silica: The influence of pore size, pore volume and mesophases |
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| Authors: | Yongjun Lü Yanglong Guo Yanqin Wang Xiaohui Liu Yunsong Wang Yun Guo Zhigang Zhang Guanzhong Lu |
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| Affiliation: | aLab for Advanced Materials, Research Institute of Industrial Catalysis, East China University of Science and Technology, Shanghai 200237, PR China |
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| Abstract: | Penicillin G acylase (PGA) was immobilized on KIT-6 and SBA-15 with different pore sizes to study the influence of pore size, pore volume and mesophases on the immobilized enzyme (IME) activity. The results show that the pore size, pore volume, mesophase and surface area have an obvious influence on the IME activity, of which the pore size is the most important factor. The activity of PGA immobilized on KIT-6/130 reaches to 3522 IU/g by dry support, which is higher than that ever reported for carrier-bound penicillin G acylase A.I. Kallenberg, F. van Rantwijk, R.A. Sheldon, Adv. Synth. Catal. 347 (2005) 905–926]. Because of the covalent immobilization, the operational stability of IME increases. |
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| Keywords: | Penicillin G acylase (PGA) Immobilization Mesoporous silica Pore size Mesophase |
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