不同生物膜载体下MBBR中微生物群落变化特征

以沸石粉和聚氨酯海绵为原料制备新型生物膜载体,对比分析了投加新型载体和传统聚氨酯海绵载体的移动床生物膜反应器(MBBR)的微生物群落结构及功能性脱氮菌属的演替变化特征。结果表明,微生物在新型载体和传统聚氨酯海绵载体上的生长速率分别为0. 009 5和0. 006 5 g/(g载体·d)。通过对微生物群落的分析,两种生物膜载体上的菌群存在较大的差异性,但Proteobacteria和Bacteroidetes在整个试验过程中是最占优势的两种菌:Shannon x ACE和Cliaol等指数表明新型载体上微生物多样性更加丰富稳定,并且存在较多的反硝化功能性菌属(Sphaerotilus、Flavobacterium和Thermomonas等),从而提高了 MBBR工艺的脱氮性能。

Characteristics of Microbial Communities in MBBR with Different Biofilm Carriers

New biofilm carrier was prepared using zeolite powders and polyurethane sponges as raw materials.The succession changes in microbial community and functional denitrification bacteria in the moving bed biofilm reactor(MBBR)with new biofilni carriers and traditional polyurethane sponge carriers were compared and analyzed.The results showed that the growth rates of microorganisms on the new biofilm carriers and the traditional polyurethane sponge carriers were 0.009 5 g/(g·d)and 0.006 5 g/(g·d),respectively.The analysis of microbial community showed great differences between the two types of biofilm carriers;however,Proteobacteria and Bacteroidetes were the two dominant bacteria in the whole experiment process.Shannon,ACE,and Chaol indices showed that the microbial diversity on the new biofilni carrier was more abundant and stable,and there were more denitrification functional bacteria(Sphaerotilus,Flavobacterium,Thermomonas,etc.)on the new biofilm carrier,which improved the nitrogen removal performance in the MBBR process.