| 榆干离褶伞菌丝体中溶栓酶的分离纯化 |
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| 引用本文： | 沈明花,金在城,朴世恩,金成俊.榆干离褶伞菌丝体中溶栓酶的分离纯化[J].食品研究与开发,2007,28(8):41-43. |
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| 作者姓名： | 沈明花 金在城 朴世恩 金成俊 |
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| 作者单位： | 1. 延边大学医学部分子生物学实验室,吉林,延吉,133000
2. 韩国朝鲜大学校自然科学大学,韩国,光州,501-759 |
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| 摘 要： | 采用离子交换层析、凝胶过滤层析及快速蛋白液相色谱等蛋白质分离纯化技术,从榆干离褶伞菌丝体中分离纯化了一种溶栓酶。实验结果表明,其分子量约为50ku,比活性为750.6Unit/mg,活性得率为12.5%。榆干离褶伞很有潜力成为治疗血栓的新的药物来源。
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| 关 键 词： | 榆干离褶伞 溶栓酶 纯化 |
| 修稿时间： | 2006-12-05 |
PURIFICATION OF FIBRINOLYTIC ENZYME FROM CULTURED MYCELIA OF LYOPHYLLUM ULMARIUM |
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| SHEN Ming-hua,JIN Jae-sung,PARK Se-eun,KIM Sung-jun.PURIFICATION OF FIBRINOLYTIC ENZYME FROM CULTURED MYCELIA OF LYOPHYLLUM ULMARIUM[J].Food Research and Developent,2007,28(8):41-43. |
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| Authors: | SHEN Ming-hua JIN Jae-sung PARK Se-eun KIM Sung-jun |
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| Affiliation: | 1. Medical College Yanbian University, Yanji 133000,Jilin,China;2. Department of Natural Science, Chosun University, Gwang-Ju 501-759, Korea |
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| Abstract: | A Fibrinolytic enzyme was purified from the cultured mycelia of Lyophyllum ulmarium by ion exchange chromatography followed by gel filtration and fast protein liquid chromatography. The apparent molecular mass of purified enzyme was estimated to be 50 kDa, specific activity was 750.6 Unit/mg and the recovery was 12.5 %. The mycelia of Lyophyllum ulmarium may thus represent a potential source of new therapeutic agents to treat thrombosis. |
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| Keywords: | Lyophyllum ulmarium fibrinolytic enzyme purification |
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