Improved preservation of fine structure of deep-sea microorganisms by freeze-substitution after glutaraldehyde fixation |
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| Authors: | Yamaguchi Masashi Namiki Yuichi Okada Hitoshi Uematsu Katsuyuki Tame Akihiro Maruyama Tadashi Kozuka Yoshimichi |
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| Affiliation: | Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan. yama@faculty.chiba-u.jp |
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| Abstract: | A method was proposed for improving preservation of ultrastructures of deep-sea microorganisms by using rapid-freeze freeze-substitution after glutaraldehyde fixation. This method produced clear high-resolution images of cells appearing in their natural state, close to the quality of images obtained by rapidly freezing freeze-substituted specimens of living cells. The method may be useful for observing any microorganism when rapid freezing of living samples is difficult and only glutaraldehyde fixation can be carried out. |
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