Chitosan Polymer as Bioactive Coating and Film against Aspergillus niger Contamination |
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Authors: | Issam Sebti Adele Martial-Gros Adele Carnet-Pantiez Stephane Grelier Veronique Coma |
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Affiliation: | Authors Sebti, Martial-Gros, and Carnet-Pantiez are with Laboratoire de Recherche en Génie Industriel Alimentaire, Univ. Claude Bernard Lyon I, IUTA-Dépt. Génie Biologique, Bourg-en-Bresse, France.;Author Grelier and Coma are with Laboratoire de Chimie des Substances Végétales, CRCM, Univ. Bordeaux 1, 351 cours de la Libération, 33405 Talence, France. Direct inquiries to author Coma (E-mail: ). |
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Abstract: | ABSTRACT: The inhibitory activity of chitosan-based edible coatings and films was assessed against the Aspergillus niger food pathogen and deterioration microorganism. Spore-counting assays showed an almost total inhibition of A. niger growth when either film-forming solution or film were used at a low concentration of chitosan (0.1% w/v). Epifluorescence microscopic results showed the action of chitosan on the relative proportion of RNA compared with DNA. The water vapor permeability (WVP) of chitosan film was relatively low compared with the poor moisture barrier of some polysaccharide films. Moreover, a coating with chitosan film on an agar gel, used as a food model, induced a 30% reduction in water loss. These results showed potential applications of chitosan-based films as bioactive packaging with properties to limit the food dehydration phenomenon. |
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Keywords: | chitosan antimicrobial film or coating Aspergillus niger epifluorescence |
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