| L-异亮氨酸发酵培养基的响应面法优化 |
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| 引用本文： | 陈宁,常高峰,张克旭. L-异亮氨酸发酵培养基的响应面法优化[J]. 食品与发酵工业, 2004, 30(2): 33-37 |
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| 作者姓名： | 陈宁 常高峰 张克旭 |
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| 作者单位： | 天津科技大学食品科学与生物工程学院,天津,300222 |
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| 基金项目： | 天津科技大学自然基金资助 |
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| 摘 要： | 借助于SAS软件 ,采用Plackett Burman试验设计法及响应面法分析 ,对L 异亮氨酸产生菌BrevibacteriumflavumTC 2 1进行了发酵培养基的优化研究。在初始发酵培养基的基础上寻优 ,优化后的发酵培养基使TC 2 1菌株的L 异亮氨酸产率提高了 2 2 5 2 %。
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| 关 键 词： | SAS 发酵 L-异亮氨酸 |
| 修稿时间： | 2003-09-17 |
Optimization of L-isoleucine Fermentation Medium Using Response Surface Methodology |
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| Chen Ning Chang Gaofeng Zhang Kexu. Optimization of L-isoleucine Fermentation Medium Using Response Surface Methodology[J]. Food and Fermentation Industries, 2004, 30(2): 33-37 |
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| Authors: | Chen Ning Chang Gaofeng Zhang Kexu |
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| Abstract: | The static culture media of Brevibacterium flavum TC-21 producing L-isoleucine was optimized using SAS software combined with the Plackett-Burman design and Response surface alalysis method. The optimum area was optimized base on initial flask-shaking batch fermentation medium. It is found that the L-isoleucine yield of TC-21 was increased by 22.52% using the optimized medium. |
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| Keywords: | SAS fermentation L-isoleucine |
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