牙鲆淋巴囊肿病的诊断技术研究

使用本实验室设计的PCR引物，在标记反应体系下，通过PCR反应合成了碱基数为172bp的牙鲆（Paralichthys olivaceus）囊肿病（Lymphocysits disease,LCD）的地高辛标记探针，在其琼脂糖凝胶电泳图172bp处有明显的亮带，由软件分析得出，合成探针的浓度为500ng/μl，此结果证明，该产物确是所需的探针，且具有很好的合成效率。通过实验证明本文采用的核酸探针-杂交诊断方法可以有效地定性和定位LCDV。实验结果还提示。LCDV是通过血液在牙鲆体内传播的，并可能同时存在垂直和水平两种传播方式。

The Studies on Diagnositic Method of Dot-blot and in situ Hybridization for Lymphocystis Disease of Cultured Paralichthys olivaceus

A DIG-labeling nucleic acid probe for paralichthys olivaceus lymphosystis disease Virus(LCDV)has been developed with PCR method.With the PCR primer designed by our laboratory,the apparent light bands at 176bp of the agrose eletrophoresis of DIG-labeling DNA probe proved that the syntheisized product is the expecteel probe,and the probe has high productivity.According to the diagnosis results of nucleic acid probe-hybridization, LCDV is inferred to propagate via blood within Paralichthys olivaceus, in addition, the possibility of parallel and orthogonal propagation also exist.