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| 介孔分子筛MCM-48固定化木瓜蛋白酶性质的研究 | |
| 引用本文： | 赵炳超,马润宇,石波.介孔分子筛MCM-48固定化木瓜蛋白酶性质的研究[J].食品与发酵工业,2005,31(10):60-63. |
| 作者姓名： | 赵炳超 马润宇 石波 |
| 作者单位： | 1. 北京化工大学生命科学与技术学院,北京,100029;中国农业科学院饲料研究所,北京,100081 2. 北京化工大学生命科学与技术学院,北京,100029 3. 中国农业科学院饲料研究所,北京,100081 |
| 摘 要： | 以介孔分子筛MCM48为载体、戊二醛作交联剂对木瓜蛋白酶进行了固定化。实验结果表明,与游离酶相比,固定化酶的热稳定性有了显著的提高,其半衰期达到了2500min以上,是游离酶(80min)的30倍以上。固定化酶的pH稳定性和储藏稳定性也有了明显改善。此外,固定化酶还具有很好的操作稳定性,经过12d的连续酶解,酶活力仍保持在初始酶活力的70%以上。 |
| 关 键 词： | 固定化 木瓜蛋白酶 介孔分子筛MCM-48 戊二醛 |
| 收稿时间： | 05 20 2005 12:00AM |
| 修稿时间： | 2005年5月20日 |
Immobilization of Papain on Mesoporous Molecular Sieve MCM-48 |
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| Zhao Bingchao,Ma Runyu,Shi Bo.Immobilization of Papain on Mesoporous Molecular Sieve MCM-48[J].Food and Fermentation Industries,2005,31(10):60-63. | |
| Authors: | Zhao Bingchao Ma Runyu Shi Bo |
| Affiliation: | 1,College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China;2,Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China |
| Abstract: | The present work dealt with the immobilization of papain on the mesoporous molecular sieve MCM-48 with the aid of a coupling agent, glutaraldehyde. The thermostability of immobilized enzyme was greatly improved. The half-life of the immobilized enzyme was more than 2 500 min, which was significantly higher than that of free enzyme (about 80 min). The influence of pH on immobilized enzyme activity was less than on free enzyme. The immobilized enzyme also showed preferable storage stability as compared with free enzyme. Moreover, the immobilized enzyme exhibited good operational stability, its activity was still over 70% based on the initial activity after 12-day continuous operation. |
| Keywords: | immobilization papain mesoporous molecular sieve MCM-48 glutaraldehyde |
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