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Studies on toxicity of multi-walled carbon nanotubes on Arabidopsis T87 suspension cells
Authors:Chun Lin   Bunshi Fugetsu   Youbo Su  Fumio Watari
Affiliation:aGraduate School of Environmental Science, Hokkaido University, Sapporo 060-0810, Japan;bCollege of Resources and Environment, Yunnan Agricultural University, Kunming 650201, PR China;cGraduate School of Dental Medicine, Hokkaido University, Sapporo 060-8586, Japan
Abstract:The possible toxicities of agglomerates of multi-walled carbon nanotubes (MWCNTs) toward plant cells were evaluated. Arabidopsis T87 suspension cells were used as the model cells and decreases in values of cell dry weights, cell viabilities, cell chlorophyll contents, and superoxide dismutase (SOD) activities were seen for the cells cultured in media containing the agglomerates of MWCNTs, indicating the agglomerates of MWCNTs are toxic to the Arabidopsis T87 suspension cells. Moreover, the toxicities increased sharply as the diameters of the agglomerates of the MWCNTs became smaller. A concept based on the hypersensitive response is suggested to explain the possible toxic mechanism induced by the MWCNTs.
Keywords:Multi-walled carbon nanotubes   White asbestos   Arabidopsis T87 suspension cells   Cell dry weights   Cell viabilities   Cell chlorophyll contents   Superoxide dismutase
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