透明质酸在电-膜分离过程中的迁移规律

对电-膜分离技术用于透明质酸分离过程的可行性进行了初步研究. 着重研究了透明质酸在电场作用下的行为，并考察了料液中透明质酸浓度、溶液pH值、离子强度、电流强度等因素对迁移过程的影响. 结果表明，透明质酸透过膜的速度在一定范围内与料液的浓度和电流强度成正比，在pH 7~9范围内透明质酸的电迁移率基本保持恒定. 膜孔径对迁移速度没有明显影响，单位膜面积的产量也与电流密度成正比. 该方法能有效地将透明质酸与蛋白质分离.

Behavior of Hyaluronic Acid in Electro-Membrane Separation

An electro-membrane separation technique was introduced to extract hyaluronic acid from fermentation broth. The present studies were focused on the behavior of hyaluronic acid under electrical field and the effects of hyaluronic acid concentration, pH, ion strength and electric current intensity on the extraction. The results show that the extraction rate of hyaluronic acid is proportional to the concentration and the electric current. The electrophoresis mobility of hyaluronic acid is kept constant within pH 7-9, and the extraction of hyaluronic acid is independent of pore size. The separation experiment with fermentation broth shows that hyaluronic acid can be fully separated from proteins in broth.