不同培养条件对红曲霉产红曲色素的研究

本研究主要针对发酵液培养基中不同碳源、氮源、pH以及装液量分别进行单因素五水平的试验,确定三个较好的水平,再进行四因素三水平正交试验以确定红曲霉产红曲色素的最佳条件.在单因素试验中,碳源以玉米粉、淀粉、麦芽糖较好;氮源以硝酸钠、大豆粉、氯化铵较好;pH值以pH3.8、5.4、7.0较好;装液量以250ml三角瓶装100、125、150ml较好.通过四因素三水平正交试验,确定出红曲霉产红曲色素的最佳培养条件为A3BtC1D3,即麦芽糖、大豆粉、pH3.8、装液量150ml/250ml组合最佳.

Study on Liquid Fermentation of Monascus ruber Pigment at Different Culture Conditions

With carbon, nitrogen, pH value and fermentation liquid volume in flask as test factors, producing conditions of pigment by Monascus rubber were optimized by L9(34) orthogonal test. Signal test results showed that corn powder, maltose and starch are the better carbons; NaNO3, soya powder, NH4Cl are the better nitrogen; pH3.8, pH5.4, pH7.0 are the better pH values of substrate; and 100 ml/250 ml, 125 ml/250 ml, 150 ml/250 ml are the better fermentation liquid volumes. Orthogonal test results showed that A3B1C1D3 is the optimum conditions combination for producing Monascus rubber pigments, that is to say, maltose as carbon, soya powder as nitrogen, pH3.8 and 150 ml/250 ml fermentation liquid volume in flask are the optimum conditions.