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| 高效液相色谱法测定山西老陈醋中的川芎嗪 | |
| 引用本文： | 张少飞,李婷,彭晓光,朱张洪基,黄登宇.高效液相色谱法测定山西老陈醋中的川芎嗪[J].食品工程,2009(2):43-45. |
| 作者姓名： | 张少飞 李婷 彭晓光 朱张洪基 黄登宇 |
| 作者单位： | 1. 山西大学生物技术研究所,太原,030006 2. 山西省生物研究所,太原,030006 3. 山西大学生物技术研究所,太原,030006;山西省食品药品监督管理局,太原,030012 |
| 基金项目： | 山西省科技工业攻关项目 |
| 摘 要： | 实验通过酸与有机溶剂交替萃取，建立高效液相色谱法（HPLC）测定食醋中川芎嗪的方法。将山西老陈醋样品萃取液浓缩后进行HPLC分析，川芎嗪标准溶液在1μg／mL～100μg／mL内与峰面积线性良好，回归方程：A=55．059C＋0．7339，R^2=0．9999。精密度试验RSD=0．577％，重现性试验RSD=2．744％，平均回收率=85．44％，RSD为7．11％。该方法样品处理简单，分离效果好，测定结果准确度高，可用于山西老陈醋中川芎嗪含量的定量分析。 |
| 关 键 词： | 山西老陈醋 川芎嗪 高效液相色谱 |
Determination of the ligustrazine in the Shanxi mature vinegar by high efficiency liquid chromatography |
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| ZHANG Shao-fei,LI Ting,PENG Xiao-guang,ZHU ZHANG-HongjI,HUANG Deng-yu.Determination of the ligustrazine in the Shanxi mature vinegar by high efficiency liquid chromatography[J].Food Engineering,2009(2):43-45. | |
| Authors: | ZHANG Shao-fei LI Ting PENG Xiao-guang ZHU ZHANG-HongjI HUANG Deng-yu |
| Affiliation: | Institute of Biotechnology;Shanxi University;Taiyuan 030006;China;Shanxi Institute of Biology;China;Food and Drug Administration;Shanxi Province;Taiyuan 030012;China |
| Abstract: | This experiment used the extraction method by acid and organic solvent alternatively, established the method for determination of the ligustrazine in the Shanxi mature vinegar by high efficiency liquid chromatography (HPLC). The sample of the Shanxi mature vinegar was analyzed by HPLC after concentrated. The titrating solu-tion of ligustrazine has good correlation with the peak area within the range of 1μg/mL～100 μ g/mL, regres-sion equation: A=55.059 C+0.7339, R2=0.9999. RSD of degree of accuracy test is 2.744%, the average coefficient of recovery is 85.44% ,RSD is 7.11%. This method re-quires simple treatment of the sample, has good separa-tive efficiency and accuracy of determination,can beused for the determination of the ligustrazine in theShanxi mature vinegar. |
| Keywords: | Shanxi mature vinegar ligustrazine HPLC |
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