| 蓝莓酒中11种酚酸的高效液相色谱测定 |
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| 引用本文： | 翁芳华,陈建业,温鹏飞,黄卫东. 蓝莓酒中11种酚酸的高效液相色谱测定[J]. 食品科学, 2006, 27(9): 223-226 |
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| 作者姓名： | 翁芳华 陈建业 温鹏飞 黄卫东 |
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| 作者单位： | 中国农业大学食品科学与营养工程学院葡萄与葡萄酒研究中心 |
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| 摘 要： | 实验采用LiChrospher100RP-18e色谱柱(250×4.0mmID,5μm),以甲醇:乙酸:水溶液为流动相,流速为1.0ml/min梯度洗脱,SPD-6AV紫外检测器,30℃柱温,紫外检测波长为280nm。建立了一种新的同时测定蓝莓酒中11种酚酸含量的高效液相色谱法,并测定了蓝莓酒发酵样中酚酸的含量。
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| 关 键 词： | 高效液相色谱 蓝莓酒 酚酸 |
| 文章编号： | 1002-6630（2006）09-0223-04 |
| 收稿时间： | 2005-10-26 |
| 修稿时间： | 2005-10-26 |
Detection of 11 Phenolic Acids of Blueberry Wine by High Performance Liquid Chromatography |
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| WENG Fang-hua,CHEN Jian-ye,WEN Peng-fei,HUANG Wei-dong. Detection of 11 Phenolic Acids of Blueberry Wine by High Performance Liquid Chromatography[J]. Food Science, 2006, 27(9): 223-226 |
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| Authors: | WENG Fang-hua CHEN Jian-ye WEN Peng-fei HUANG Wei-dong |
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| Affiliation: | Centre for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China |
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| Abstract: | It’s a new method for simultaneously determination of 11 phenolic acids in blueberry wines by high performance liquid chromatography was studied. Chromatographic separation was performed with LiChrospher 100RP-18e(250×4.0mmID,5μ m) by using the mobile phase gradient elution of methanol-acetic acid mixture at 30℃, flow rate at 1.0ml/min and detection wavelength at 280nm. It was successful to separate the 11 phenolic acids with a high recoveries of 11 phenolic acids (>89%), also a lower detection limit. The content of 11 phenolic acids in blueberry wines of different manufactures were also determined. |
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| Keywords: | high performance liquid chromatography(HPLC) blueberry wine phenolic acid |
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