BAK和BAG-1蛋白在结直肠癌中的表达及其意义

目的探讨BAK和BAG-1蛋白在结直肠癌中的表达及其意义。方法采用免疫组化SP法对80例结直肠癌、40例结直肠腺瘤及25例癌旁正常结直肠黏膜组织中BAK和BAG-1蛋白的表达进行检测,并分析其与结直肠癌临床病理特征之间的相关性。结果 BAK和BAG-1蛋白在结直肠癌中过度表达,表达率分别为72.5%(58/80)和80%(64/80),且BAG-1蛋白与肿瘤的分化程度呈负相关,BAK蛋白与肿瘤的分化程度呈正相关。有淋巴结转移的结直肠癌,其BAG-1蛋白的表达率为96.7%(29/30),无淋巴结转移的结直肠癌,其BAG-1蛋白的表达率为70%(35/50),两者相比差异有统计学意义(P<0.01)。结论 BAK和BAG-1蛋白的过度表达与肿瘤的分化程度密切相关,且BAG-1蛋白的过度表达还与肿瘤的淋巴结转移密切相关;结直肠癌的发生机制可能涉及BAK和BAG-1调节环路中多个基因的异常。

Significance of Expressions of BAK and BAG-1 Proteins in Colorectal Carcinoma

Objective To investigate the significance of expressions of BAK and BAG-1 proteins in colorectal carcinoma.Methods The expressions of BAK and BAG-1 proteins in 80 colorectal carcinoma specimens,40 colorectal adenomas specimens and 25 adjacent normal colorectal tissue specimens were determined by immunohistochemical SP method,based on which the relationship between the expressions and clinical pathological characters of colorectal carcinoma was analyzed.Results Both BAK and BAG-1 proteins were over-expressed in colorectal carcinoma,with positive rates of 72.5%(58 /80)and 80%(64 /80)respectively.The expression level of BAG-1 was negatively related,while that of BAK was positively related to the differentiation degree of tumor.The positive rates of BAG-1 in colorectal carcinoma with and without metastasis in lymph node were 96.7%(29 /30)and 70%(35 /50) respectively,which showed significant difference(P < 0.01).Conclusion The overexpressions of BAK and BAG-1 were closely related to the differentiation degree,while that of BAG-1 to the metastasis in lymph node of colorectal carcinoma.The mechanism of colorectal carcinogenesis might be involved in the abnormality of several genes in the regulatory pathway of BAK and BAG-1.