气相色谱—串联质谱法测定卷烟主流烟气中苯并[a]芘及其同分异构体含量

应用气相色谱—串联质谱技术(GC-MS/MS)建立一种测定卷烟主流烟气中苯并[a]芘及其同分异构体苯并[b]荧蒽(B[b]F)、苯并[k]荧蒽(B[k]F)、苯并[j]荧蒽(B[j]F)、苯并[j]醋蒽烯(B[j]A)、苯并[e]醋蒽烯(B[e]A)、苯并[e]芘(B[e]P)、苝(PER)含量的方法,并以内标法定量。结果表明,目标物的检出限、定量限分别为0.056 6~0.181 7,0.188 6~0.605 5 ng/支,线性相关系数达0.998以上;日内重复性和日间重现性分别为1.91%~3.97%,2.54%~4.46%;3种不同添加浓度下,加标回收率为92.60%~107.60%。表明试验方法操作简便、重复性好、受环境和人为因素干扰较小,可作为一种卷烟主流烟气中苯并[a]芘及其同分异构体含量的快速检测方法加以推广。

Simultaneous determination of benzo[a]pyrene and its structural isomers in mainstream cigarette smoke by GC-MS/MS

In this paper, employed an inter-standard quantitative method for determination of benzo[a]pyrene and its structural isomers (B[b]F, B[k]F, B[j]F, B[j]A, B[e]A, B[e]P, PER) in mainstream cigarette smoke by GC-MS/MS. The results showed that the LODs and the LOQs of target PAHs could achieve 0.056 6~0.181 7 and 0.188 6~0.605 5 ng/cig, respectively, while the linear correlation efficiencies are larger than 0.998 for all PAHs. The Intra-assay and Inter-assay precisions measured by 15 repeat runs are 1.91%~3.97% and 2.54%~4.46%, respectively. And with three different added-levels, the recovery rates for the target PAHs lie between 92.60% and 107.60%. It was proved that the developed method is environmentally friendly, highly sensitive and selective.