| 玉米芯固定红曲霉产酯化酶条件的研究 |
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| 引用本文： | 郭宝禹,郝林,张英,郭昌云,张亚丽.玉米芯固定红曲霉产酯化酶条件的研究[J].酿酒,2011,38(2):29-32. |
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| 作者姓名： | 郭宝禹 郝林 张英 郭昌云 张亚丽 |
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| 作者单位： | 山西农业大学食品科学与工程学院,山西,太谷,030801 |
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| 摘 要： | 以红曲霉为研究材料,用玉米芯固定红曲霉,对载体玉米芯的质量、固定培养时间、固定培养温度、接种量、补充氮源、固定初始pH和半固体培养基的量几个因素进行实验和正交分析,得出固定化红曲霉培养的最佳条件：载体玉米芯的质量为18g,固定培养时间4d,固定培养温度30℃,接种量为18mL孢子悬浮液,以硝酸铵作为氮源,初始pH为4.0,半固体培养基的量27mL,总酯含量可达到33.44mg/100mL。
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| 关 键 词： | 固定化 红曲霉 酯化酶 |
Studies on Immobilizing Monascus with Corn Cobs that Procducting Ester Enzyme |
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| GUO Bao-yu,HAO Lin,Zhang Ying,GUO Chang-yun,ZHANG Ya-li.Studies on Immobilizing Monascus with Corn Cobs that Procducting Ester Enzyme[J].Liquor Making,2011,38(2):29-32. |
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| Authors: | GUO Bao-yu HAO Lin Zhang Ying GUO Chang-yun ZHANG Ya-li |
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| Affiliation: | GUO Bao-yu,HAO Lin,Zhang Ying,GUO Chang-yun,ZHANG Ya-li(College of Food Science and Engineering,Shanxi Agriculture University,Taigu 030801,Shanxi,China) |
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| Abstract: | As the research material,the Monascus was fixed with corn cobs.The quality of the carrier,fixed incubation time,fixed incubation temperature,inoculation,added nitrogen source,initial pH value and the amount of semi-solid medium are the experimental factors.We studied these factors and orthogonal experiment analysis,the best conditions of immobilized Monascus：the quality of the carrier 18g,fixed incubation time 4d,fixed incubation temperature 30℃,inoculation spore suspension 18mL,ammonium nitrate as a nitrogen source,initial pH 4.0,the amount of semi-solid medium 27mL.Under the conditions,the ester enzyme activity reached 33.44mg/100mL. |
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| Keywords: | immobilization Monascus ester enzyme |
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