| 巴斯德毕赤酵母PEP4基因的剔除及对基因工程菌外源蛋白质表达的影响 |
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| 引用本文： | 杨扬,赵健,石军.巴斯德毕赤酵母PEP4基因的剔除及对基因工程菌外源蛋白质表达的影响[J].食品与药品,2007,9(4):10-13. |
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| 作者姓名： | 杨扬 赵健 石军 |
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| 作者单位： | 1. 华东理工大学生物反应器工程国家重点实验室,上海,200237
2. 上海生命科学院模式生物,中心,上海,200031 |
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| 摘 要： | 目的构建蛋白酶缺失菌株,提高外源蛋白质表达的分泌率与产量。方法构建质粒pRS306/PEP4 L&R,利用基因重组原理剔除巴斯德毕赤酵母中的PEP4基因。比较基因剔除前后外源蛋白质表达量的差异。结果剔除毕赤酵母中的PEP4基因后外源蛋白质表达量平均提高9．5%。结论剔除毕赤酵母中的PEP4基因可有效地控制外源蛋白质MIP表达时的降解。
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| 关 键 词： | 巴斯德毕赤酵母 蛋白酶 PEP4基因 |
| 文章编号： | 1672-979X(2007)04-0010-04 |
| 修稿时间： | 2006年12月8日 |
Expression Optimization of Heterologous Protein in Pichiapastoris by Disrupting PEP4 Gene |
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| YANG Yang,ZHAO Jian,SHI Jun.Expression Optimization of Heterologous Protein in Pichiapastoris by Disrupting PEP4 Gene[J].Food and Drug,2007,9(4):10-13. |
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| Authors: | YANG Yang ZHAO Jian SHI Jun |
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| Abstract: | Objective To create protease-deficient mutants for improving productivity and secretion efficiency of heterologous protein expression.Methods PEP4 gene of Pichia pasioris was disrupted by transforming with pRS306/PEP4 L&R vector.Effects of the resultant PEP4 strain on heterologous protein expression were then measured by practical expression compared with the wild strain.Results By disrupting the PEP4 gene in Pichia pastoris,the productivity of heterologous protein expression increased 9.5% on average.Conclusion Disruption of PEP4 gene in Pichia pastoris is an effective method for controlling proteolytic degradation of heterologous protein MIP. |
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| Keywords: | Pichia pastoris protease PEP4 gene |
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