可再生使用的磁性纳米修饰C反应蛋白电流型免疫传感器

利用Fe3O4(核)/Au(壳)(简称GMPs)标记C反应蛋白酶标抗体(HRP-anti CRP),构建了一类新型的磁性纳米探针(HRP-anti CRP/GMPs),将其修饰在丝网印刷电极(SPCE)表面构建了可再生使用的CRP安培型酶联免疫传感器.首先将多壁碳纳米管(MCNTs) -硫堇(Thi) -Nafion复...

A renewable immunosensor for CRP based on magnetic nano particles modified screen printed electrode

A renewable amperometric immunosensor for C reactive protein(CRP)based on a novel magnetic nano probes modified on screen printed electrode(SPCE)was fabricated.The probes was prepared by horseradish peroxidase(HRP)labeled CRP antibody(HRP-anti-CRP)assembled on nano Fe3O4(core)/Au(shell)(GMPs)particles.The immunosensor was prepared by following steps.Firstly,multi-walled carbon nanotubes(MCNTs)-Thionine(Thi)-Nafion composite was prepared and dropped on the surface of SPCE to prepare the basic electrode(SPCE/MCNTs-Thi-Nafion).Secondly,the nanoprobes were introduced on the surface of the basic electrode by permanent magnet,then to prepare the immunosensor(SPCE/MCNTs-Thi-Nafion/HRP-anti-CRP/GMPs).Through one-step immunoassay format,the immunosensor was incubated with CRP solution.Under optimized conditions,the decreased current was proportional to the CRP concentration from 0.1 to 110ng/mL with a detection limit of 0.04 ng/mL at signal/noise ratio of 3,This method reduced the cost and simplified the preparation process of the immunosensor.The immunosensor can simultaneously realize separation,enrichment and determination,with high sensitivity and good stability,which would be valuable for clinical immunoassay for CRP in human serum.