Effect of n?3 fatty acid supplementation on lipid peroxidation and protein aggregation in rat erythrocyte membranes |
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Authors: | Ken Ando Kunihide Nagata Masatoshi Beppu Kiyomi Kikugawa Terue Kawabata Kyoko Hasegawa Masao Suzuki |
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Affiliation: | (1) Kagawa Nutrition University, Sakado, 350-02 Saitama, Japan;(2) Oleochemical Research Laboratory, Amagasaki, 660 Osaka, Japan;(3) School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, 192-0392 Tokyo, Japan |
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Abstract: | Human erythrocytes in the circulation undergo dynamic oxidative damage involving membrane lipid peroxidation and protein aggregation
during aging. The present study was undertaken to determine the effect of n−3 fatty acid supplementation on lipid peroxidation
and protein aggregation in the circulation and also the in vitro susceptibility of rat erythrocyte membranes to oxidative damage. Wistar male rats were fed a diet containing n−6 fatty acid-rich
safflower oil or n−3 fatty acid-rich fish oil with an equal amount of vitamin E for 6 wk. n−3 Fatty acid content in erythrocyte
membranes of rats fed fish oil was significantly higher than that of rats fed safflower oil. The degree of membrane lipid
peroxidation and protein aggregation of rats fed fish oil was not significantly higher than that of rats fed safflower oil
when the amounts of phospholipid hydroper-oxides, thiobarbituric acid-reactive substances, and detergent-insoluble protein
aggregates were measured. When isolated erythrocytes were oxidized under aerobic conditions in the presence of Fe(III), the
degree of membrane lipid peroxidation of erythrocytes from rats fed fish oil was increased to a greater extent than that of
rats fed safflower oil, whereas the degree of membrane protein aggregation of both groups was increased in a similar extent.
Hence, n−3 fatty acid supplementation did not affect lipid peroxidation and protein aggregation in membranes of circulating
rat erythrocytes, and the supplementation increased the susceptibility of isolated erythrocytes to lipid peroxidation, but
not to protein aggregation, under the aerobic conditions. If a sufficient amount of vitamin E is supplied, n−3 fatty acid
supplementation may give no undesirable oxidative effects on rat erythrocytes in the circulation. |
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