| 酸解提取胶原的研究 |
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| 引用本文： | 张琦,王进美,王卫. 酸解提取胶原的研究[J]. 西北纺织工学院学报, 2011, 0(1): 18-21 |
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| 作者姓名： | 张琦 王进美 王卫 |
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| 作者单位： | [1]西安工程大学纺织与材料学院,陕西西安710048 [2]西安工程大学科技处,陕西西安710048 |
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| 摘 要： | 以新鲜猪皮为原料,采用有机溶剂无水乙醇、异丙醇两部法除去碎猪皮液的脂肪,再用二氯甲烷抽提,完全去除碎猪皮液中的脂肪,然后用盐酸水解的方法提取胶原.在单因素实验的基础上,通过正交实验确定胶原蛋白提取的最佳条件为超声时间2h、盐酸的浓度10%、温度30℃、液比1∶3.5,在此条件下的胶原纯度达95.45%.红外光谱测试显示,胶原蛋白具有三股螺旋结构.
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| 关 键 词： | 胶原蛋白 酸处理 提取率 猪皮 |
Research on extracting collagen protein by acid |
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| ZHANG Qi,WANG Jin-mei,WANG Wei. Research on extracting collagen protein by acid[J]. Journal of Northwest Institute of Textile Science and Technology, 2011, 0(1): 18-21 |
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| Authors: | ZHANG Qi WANG Jin-mei WANG Wei |
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| Affiliation: | 1.School of Textile and Materials,Xi′an Polytechnic University,Xi′an 710048,China; 2.Science ＆ Technology Administration Department,Xi′an Polytechnic University,Xi′an 710048,China) |
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| Abstract: | Taking the fresh pig skin as material,the pig skin fat was removed by the method of two step-organic solvents and then extracted by dichloromethane.At last,the liquid of skin was hydrolyed by the method of acid treatment to get the collagen protein.The best optimization condition of hydrolyzed is as follows： the ultrasonic time is 2h,the ratio of hydrochloride is 10%,the temprature is 30℃,the liquid ratio is 1∶3.5,a good spiral structure with the single-factor experiment is obtained. |
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| Keywords: | collagen protein acid treatment yield ratio pig skin |
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