RADIOASSAY OF FOLACIN IN FOODSTUFFS |
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Authors: | PAVEL RAUCH,JAN KÁ S,FRANTISEK STREJCEK,JITKA CKERNÁ |
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Affiliation: | Department of Biochemistry and Microbiology, Institute of Chemical Technology, 16628 Prague 6, Czechoslovakia;Research Institute of Food Industry, 15038 Prague 5, Czechoslovakia |
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Abstract: | A commercial radioassay kit for folate (Radio-chemical Centre Amersham, England), produced for clinical analysis, has been tested for folacin assay in foodstuffs. The binding capacity of porcine binder was determined for different folates and compounds interfering with the microbiological assay. Recovery of standard addition of N5-methyltetrahydrofolate to the foodstuffs extracts estimated by radioassay varied from 96 to 105%. |
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