Orthogonal Light-Dependent Membrane Adhesion Induces Social Self-Sorting and Member-Specific DNA Communication in Synthetic Cell Communities |
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Authors: | Ali Heidari Oya I Sentürk Shuo Yang Alex Joesaar Pierangelo Gobbo Stephen Mann Tom F A de Greef Seraphine V Wegner |
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Affiliation: | 1. Institute of Physiological Chemistry and Pathobiochemistry University of Münster, Waldeyerstr. 15, 48149 Münster, Germany;2. Department of Physical Chemistry of Polymers, Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany;3. Laboratory of Chemical Biology and Institute for Complex Molecular Systems, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, 5612 AZ The Netherlands;4. Department of Chemical and Pharmaceutical Sciences, University of Trieste, Trieste, 34127 Italy;5. Centre for Protolife Research and Centre for Organized Matter Chemistry, Max Planck Bristol Centre for Minimal Biology, School of Chemistry, University of Bristol, Bristol, BS8 1TS UK |
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Abstract: | Developing orthogonal chemical communication pathways in diverse synthetic cell communities is a considerable challenge due to the increased crosstalk and interference associated with large numbers of different types of sender-receiver pairs. Herein, the authors control which sender-receiver pairs communicate in a three-membered community of synthetic cells through red and blue light illumination. Semipermeable protein-polymer-based synthetic cells (proteinosomes) with complementary membrane-attached protein adhesion communicate through single-stranded DNA oligomers and synergistically process biochemical information within a community consisting of one sender and two different receiver populations. Different pairs of red and blue light-responsive protein-protein interactions act as membrane adhesion mediators between the sender and receivers such that they self-assemble and socially self-sort into different multicellular structures under red and blue light. Consequently, distinct sender-receiver pairs come into the signaling range depending on the light illumination and are able to communicate specifically without activation of the other receiver population. Overall, this work shows how photoswitchable membrane adhesion gives rise to different self-sorting protocell patterns that mediate member-specific DNA-based communication in ternary populations of synthetic cells and provides a step towards the design of orthogonal chemical communication networks in diverse communities of synthetic cells. |
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Keywords: | DNA-based communication photoswitchable proteins proteinosome self-sorting synthetic cells |
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