| 采用极性及非极性溶剂从酸洗后的红法夫酵母中高效萃提虾青素的研究(英文) |
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| 引用本文： | 尹春华,杨淑珍,刘晓璐,闫海. 采用极性及非极性溶剂从酸洗后的红法夫酵母中高效萃提虾青素的研究(英文)[J]. 中国化学工程学报, 2013, 21(7): 776-780. DOI: 10.1016/S1004-9541(13)60510-8 |
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| 作者姓名： | 尹春华 杨淑珍 刘晓璐 闫海 |
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| 作者单位： | Department of Biological Science and Engineering, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China |
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| 基金项目： | Supported by the Fundamental Research Funds for the Central Universities (FRF-AS-10-001B);the National Natural Science Foundation of China(11071013) |
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| 摘 要： | method of extracting astaxanthin from Phaffia rhodozyma with various solvents after acid washing was investigated.The extraction efficiency was distinctly increased after acid washing of P.rhodozyma cells.When the concentration of HCl was 0.4 mol·L-1,the highest extraction efficiency of astaxanthin was achieved which was about three times higher than the control.Acetone or benzene as single polar or non-polar solvent was the most effective solvent in our research.With a combination of isopropanol and n-hexane(volume ratio of 2:1),the maximal extraction efficiency was achieved,approximately 60% higher than that obtained with a single solvent.The liquid-solid ratio and the extracting time were also optimized.Under the optimum extraction conditions,the extraction yield of astaxanthin exceeded 98%.
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| 关 键 词： | astaxanthin Phaffia rhodozyma acid wash extraction solvent |
| 收稿时间： | 2012-03-03 |
Efficient Extraction of Astaxanthin from Phaffia rhodozyma with Polar and Non-polar Solvents after Acid Washing |
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| YIN Chunhua,YANG Shuzhen,LIU Xiaolu and YAN Hai. Efficient Extraction of Astaxanthin from Phaffia rhodozyma with Polar and Non-polar Solvents after Acid Washing[J]. Chinese Journal of Chemical Engineering, 2013, 21(7): 776-780. DOI: 10.1016/S1004-9541(13)60510-8 |
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| Authors: | YIN Chunhua YANG Shuzhen LIU Xiaolu YAN Hai |
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| Affiliation: | Department of Biological Science and Engineering, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China |
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| Abstract: | method of extracting astaxanthin from Phaffia rhodozyma with various solvents after acid washing was investigated. The extraction efficiency was distinctly increased after acid washing of P. rhodozyma cells. When the concentration of HCl was 0.4 mol·L-1, the highest extraction efficiency of astaxanthin was achieved which was about three times higher than the control. Acetone or benzene as single polar or non-polar solvent was the most effective solvent in our research. With a combination of isopropanol and n-hexane (volume ratio of 2:1), the maximal extraction efficiency was achieved, approximately 60% higher than that obtained with a single solvent. The liquid-solid ratio and the extracting time were also optimized. Under the optimum extraction conditions, the extraction yield of astaxanthin exceeded 98%. |
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| Keywords: | astaxanthin acid wash extraction solvent |
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