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| 高效液相色谱法快速测定红酒中的白藜芦醇含量 | |
| 引用本文： | 赵丽青,方佳茂,肖向东,陈伟滨,张帆.高效液相色谱法快速测定红酒中的白藜芦醇含量[J].广东化工,2012,39(7):157-158. |
| 作者姓名： | 赵丽青 方佳茂 肖向东 陈伟滨 张帆 |
| 作者单位： | 1. 深圳大学化学与化工学院食品科学与工程系,广东深圳,518060 2. 广东环西生物科技股份有限公司,广东普宁,515343 3. 深圳大学医学院,广东深圳,518060 |
| 基金项目： | 深圳大学基础研究科研项目 |
| 摘 要： | 文章建立了高效液相色谱法测定红酒中白藜芦醇的方法,色谱柱为Agilent ZORBAX Eclipse XDB C18(4.6 mm×250 mm,5μm),以乙腈-水(35∶65,水相中含0.1%冰乙酸)为流动相,流速1 mL/min,进样量20μL,室温,检测波长300 nm,外标法定量,该方法相对标准偏差为1.18%~1.99%,线性相关系数R2为0.999,检测限为2μg/mL。在上述条件下测定了随机抽取的部分市售红酒样品的白藜芦醇含量力。 |
| 关 键 词： | 白藜芦醇 红酒 高效液相色谱法 |
Rapid Detection of Resveratrol in Red Wine by HPLC |
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| Zhao Liqing,Fang Jiamao,Xiao Xiangdong,Chen Weibin,Zhang Fan.Rapid Detection of Resveratrol in Red Wine by HPLC[J].Guangdong Chemical Industry,2012,39(7):157-158. | |
| Authors: | Zhao Liqing Fang Jiamao Xiao Xiangdong Chen Weibin Zhang Fan |
| Affiliation: | 1. Department of Food Science and Engineering, College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen 518060; 2. Guangdong Huanxi Biological Technology Co., Ltd., Puning 515343. 3. School of Medicine, Shenzhen University, Shenzhen 518060, China) |
| Abstract: | The paper focused on the conditions for resveratrol detection by HPLC. The detection conditions were shown as following： the colunm was Agilent ZORBAX Eclipse XDB C 18 （4.6 mm×250 mm, 5 ×m） column, acetonitrile-water （35 ： 65, with 0.1% acetic acid in water） as mobile phase, flow rate was 1 mL/min and the injection volume was 20 ×L, the column temperature was at room temperature and detection wavelength was 300 nm. The relative standard deviation ranges from 1.18 % to 1.99 %, linear correlation coefficient R2 was of 0.999 and the detection limit was 2 ×g/mk The resveratrol concentration of commercially available red wine samples was detected by this HPLC method. |
| Keywords: | resveratrol red wine HPLC |
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