高效液相色谱法测定腊肉中的4种氟喹诺酮类药物残留

建立了高效液相色谱法同时测定腊肉中环丙沙星、达氟沙星、恩诺沙星和沙拉沙星4种氟喹诺酮类药物残留方法。腊肉样品经过10%高氯酸溶液除蛋白、二氯甲烷提取,蒸干后,用2.00 mL流动相定容。采用Waters Symmetry C18色谱柱,用0.2%甲酸水-乙腈-甲醇作为流动相进行分离。这4氟喹诺酮种类药物的检出限为2.0~10μg/kg,线性范围为：0.02~10μg/mL,平均回收率为：76.2%~103.2%,相对标准偏差为：3.6%~7.3%。

HPLC Determine Four Kinds of Fluoroquinolones Residues in Bacon

A method for determining ciprofloxacin,danofloxacin,enrofloxacin,sarafloxacin residues in bacon was established.The protein in bacon sample was excepted by perchloric acid solution,then extracted by dichloromethane.After evaporated Constanted volume by 2.00 mL mobile phase.Chromatographic separation was achieved by using Waters Symmetry C18 column.Mobile phase was formic acid solution(0.2 %)-acetonitrile-methanol.The detection limits of these four kinds of fluoroquinolones were between 2.0~10 μg /kg,the linear range was between 0.02~10 μg/mL,the average recovery was between 76.2 %~103.2 %,the relative standard deviation was between 3.6 %~7.3 %.