Chemical Characterization and Antitumor Activities of Polysaccharide Extracted from Ganoderma lucidum |
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| Authors: | Zengenni Liang Youjin Yi Yutong Guo Rencai Wang Qiulong Hu Xingyao Xiong |
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| Affiliation: | 1.College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, China; E-Mails: (Z.L.); (Q.H.);2.College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China; E-Mail: ;3.State Key Laboratory of Sub-Health Intervention Technology, State Administration of Traditional Chinese Medicine, Changsha 410128, China; E-Mail: |
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| Abstract: | Ganoderma lucidum polysaccharide (GLP) is a biologically active substance reported to possess anti-tumor ability. Nonetheless, the mechanisms of GLP-stimulated apoptosis are still unclear. This study aims to determine the inhibitory and apoptosis-inducing effects of GLP on HCT-116 cells. We found that GLP reduced cell viability on HCT-116 cells in a time- and dose-dependent manner, which in turn, induced cell apoptosis. The observed apoptosis was characterized by morphological changes, DNA fragmentation, mitochondrial membrane potential decrease, S phase population increase, and caspase-3 and -9 activation. Furthermore, inhibition of c-Jun N-terminal kinase (JNK) by SP600125 led to a dramatic decrease of the GLP-induced apoptosis. Western blot analysis unveiled that GLP up-regulated the expression of Bax/Bcl-2, caspase-3 and poly (ADP-ribose) polymerase (PARP). These results demonstrate that apoptosis stimulated by GLP in human colorectal cancer cells is associated with activation of mitochondrial and mitogen-activated protein kinase (MAPK) pathways. |
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| Keywords: | human colon cancer cells apoptosis mitochondria caspase MAPK |
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