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| 葛根黄酮提取物对HL-60细胞bc1-2和bax基因表达的影响 | |
| 引用本文： | 袁怀波,凌庆枝,糜漫天,陈宗道. 葛根黄酮提取物对HL-60细胞bc1-2和bax基因表达的影响[J]. 食品科学, 2005, 26(11): 241-243 |
| 作者姓名： | 袁怀波 凌庆枝 糜漫天 陈宗道 |
| 作者单位： | 1. 合肥工业大学生物与食品学院,安徽,合肥,230009 2. 第三军医大学营养卫生学教研室,重庆,400038 3. 西南农业大学食品科学学院,重庆,400716 |
| 摘 要： | 目的：探讨葛根黄酮提取物对HL-60细胞bcl—2和bax基因表达的影响。方法：用RT—PCR和蛋白质免疫印迹技术检测葛根黄酮提取物、葛根素和大豆甙元处理HL-60后，对bcl—2和bax基因的mRNA和蛋白表达的影响。结果：葛根黄酮提取物、葛根素和大豆甙元处理HL-60细胞后能够上调bax mRNA表达水平，下调bcl-2蛋白表达和上调Bax蛋白表达，且随着处理时间的延长其诱导下调和上调能力增强。结论：葛根黄酮提取物诱导HL-60细胞凋亡与其下调bcl-2mRNA和蛋白表达水平和其上调bax mRNA和蛋白表达水平有关。 |
| 关 键 词： | 葛根黄酮 bcl-2 bax HL-60细胞 凋亡 |
| 文章编号： | 1002-6630（2005）11-0241-03 |
| 收稿时间： | 2004-11-11 |
| 修稿时间： | 2004-11-11 |
Effect of Kudzu Flavonoids Extracts on Expression of bcl-2 and Bax Gene in HL-60 Cell |
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| YUAN Huai-bo,LING Qing-zhi,MI Man-tian,CHEN Zong-dao. Effect of Kudzu Flavonoids Extracts on Expression of bcl-2 and Bax Gene in HL-60 Cell[J]. Food Science, 2005, 26(11): 241-243 | |
| Authors: | YUAN Huai-bo LING Qing-zhi MI Man-tian CHEN Zong-dao |
| Affiliation: | 1. College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009,China; 2. Department of Nutrition and Food Hygene, Military and Medical University, Chongqing 400038,China; 3.College of Food Science, Southwest Agricultural University, Chongqing 400716,China |
| Abstract: | Objective: To study the effect of kudzu flavonoids extracts on the expression of bcl-2 and bax gene in HL-60 cells.Methods: To use RT-PCR and western blotting technology to detect the mRNA and protein expression level of bcl-2 and bax gene in the HL-60 cells treated by kudzu flavonoids extracts, puerarin and daidzein. Results: In a time-dependent manner, kudzu flavonoids extracts, puerarin and daidzein increased the bax mRNA and protein expression level and lowered the bcl-2 protein expression level. Conclusion: The ability of kudzu flavonoids extracts could induce HL-60 cells apoptosis in relation to lower the bcl-2 mRNA and protein expression level and increase the bax mRNA and protein expression level. |
| Keywords: | kudzu flavonoids bcl-2 bax HL-60 cells apoptosis |
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