酶法制备葛根纤维低聚糖

以葛根膳食纤维为原料，采用酶法制备功能性纤维低聚糖，并采用高效阴离子交换色谱法测定所制备样品的糖分组成。研究酶制剂、酶解时间和酶用量对纤维低聚糖组分及含量的影响。结果表明：选择β-葡聚糖酶制备纤维低聚糖优于纤维素酶，酶解产物主要成分为葡萄糖、纤维二糖和纤维三糖。β-葡聚糖酶法制备纤维低聚糖，酶解时间2h、酶用量3U/g时所得酶解产物中纤维二糖和纤维三糖所占比例均较高，纤维低聚糖得率为11.8g/100g。

Enzymatic Preparation of Cello-oligosaccharides from Radix Puerariae Fiber

An enzymatic method for the preparation of cello-oligosaccharides from Radix Puerariae fiber was developed,and HPLC was employed to determine the sugar composition of cello-oligosaccharide extracts.The effects of enzyme type and dose as well as hydrolysis time on cello-oligosaccharide composition and contents were explored.The results showed that β-glucanase was more suitable for the preparation of cello-oligosaccharides than cellulase.Hydrolysates consisting of mainly glucose,cellobiose and cellotriose were obtained using β-glucanase.The hydrolysate obtained after 2 h of hydrolysis with 3 U/g β-glucanase contained a high proportion of cellobiose and cellotriose and the yield of cello-oligosaccharides was 11.8 g/100 g under these conditions.