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| 固定化磷脂酶用于大豆油脱胶的研究 | |
| 引用本文： | 卞清德,马英昌,罗淑年,于殿宇,梁海峰,侯潇.固定化磷脂酶用于大豆油脱胶的研究[J].中国油脂,2009,34(7). |
| 作者姓名： | 卞清德 马英昌 罗淑年 于殿宇 梁海峰 侯潇 |
| 作者单位： | 1. 九三粮油工业集团有限公司,哈尔滨,150090 2. 东北农业大学,哈尔滨,150030 3. 陕西省乾县临平中学,陕西,乾县,713304 |
| 基金项目： | 黑龙江省科技攻关计划项目"大豆综合利用关键技术研究与开发" |
| 摘 要： | 将海藻酸钠-壳聚糖固定化磷脂酶用于水化大豆油脱胶,通过单因素实验和旋转正交实验,确定最佳脱胶条件为:固定化酶添加量0.012%,起始pH 5.8,反应温度58 ℃,反应时间4 h.在最优条件下进行脱胶实验,测定固定化酶法脱胶油中的磷含量为3.85 mg/kg,脱胶效果较好.同时,固定化磷脂酶热稳定性提高,对反应环境的耐受力明显增强,使脱胶反应条件更易控制,是一种非常有效的脱胶方法. |
| 关 键 词： | 大豆油 固定化磷脂酶 非水化磷脂 脱胶 |
Degumming of soybean oil by immobilized phospholipase |
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| BIAN Qingde,MA Yingchang,LUO Shunian,YU Dianyu,LIANG Haifeng,HOU Xiao.Degumming of soybean oil by immobilized phospholipase[J].China Oils and Fats,2009,34(7). | |
| Authors: | BIAN Qingde MA Yingchang LUO Shunian YU Dianyu LIANG Haifeng HOU Xiao |
| Abstract: | The sodium alginate-chitosan immobilized phospholipase(PLA) was used for degumming of soybean oil. Through single factor test and orthogonal test, the optimal reaction conditions were determined as follows:immobilized enzyme dosage 0.012%, initial pH 5.8, reaction temperature 58 ℃, reaction time 4 h. Under the optimal conditions, the phosphorus content reduced to 3.85 mg/kg in degummed soybean oil. In addition, the thermal stabilities and the tolerance to the reaction conditions of immobilized PLA were enhanced, and the degumming conditions in practical production were easy to control. |
| Keywords: | soybean oil immobilized PLA nonhydratable phospholipids degumming |
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