放射损伤对骨髓基质细胞支持小鼠粒-巨噬系祖细胞生长的影响

为阐明放射损伤时骨髓造血基质细胞支持粒－巨噬系祖细胞造血能力的变化。采用小鼠骨髓基质细胞体外融合培养和粒－巨噬系祖细胞集落（CFU－GM）培养法，观察5Gy照射对基质细胞支持小鼠CFU－GM生长的影响。结果表明，经5Gy照射后，骨髓基质细胞支持正常粒－巨噬系祖细胞造血的能力明显下降，以伤后3－5d最为显著，伤后10d仍未恢复至正常；当骨髓基质细胞和粒－巨噬系祖细胞均受到致伤因素作用时，照射组的CFU－GM形成能力下降更为明显，至正常对照组的10％以下。说明放射损伤后骨髓基质细胞的功能受到了不同程序的损伤，因此，在治疗由此所引起造血功能障碍的同时，应加强促进基质细胞修复的措施，可能会取得更满意的疗效。

EFFECT OF MOUSE BONE MARROW STROMAL CELLS ON THE GROWTH OF GRANULOCYTE-MACROPHAGE PROGENITOR CELLS FROM MICE WITH RADIATION INJURY

To investigate the effect of mouse marrow stromal cells on the growth of granulocyte-macrophage progenitor cells (CFU-GM) from mice with radiation injury. Semi-solid culture of CFU-GM on the confluent adherent stromal cell layers of marrow cells was adopted. The experimental data demonstrated that the numbers of nomal marrow CFU-MGM formed on stromal cell layers from mice with radiation injury were significantly reduced as compared with those from normal mice within 10d, especially at 3th and 5th day after injury. The supporting function of bone marrow stromal cells on the growth of CFU-GM was impaired under the irradiation conditions of which must not be neglected in clinical treatment of radiation-induced hematopoiesis dysfunction.