Development of a quantitative immuno-affinity test column assay for on-site screening of clindamycin residues in milk |
| |
| Affiliation: | 1. Gubra, Hørsholm, Denmark;2. Department of Human Nutrition, University of Copenhagen, Frederiksberg, Denmark;3. Department of Biomedical Sciences, School of Veterinary Medicine, Cornell University, Ithaca, NY, USA;4. Department of Molecular Biosciences, University of California, Davis, CA, USA;1. Laboratory of Pharmacology, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan;2. Department of Physiology, School of Allied Health Sciences, Kitasato University, Sagamihara 252-0373, Japan |
| |
| Abstract: | A sensitive and specific monoclonal antibody against clindamycin (CLIN) was produced and used to develop an immuno-affinity test column (IATC) assay for on-site screening of CLIN residues in milk. The qualitative limit of detection of the IATC assay, estimated as 1.0 μg L?1 by visual detection, was sufficient to measure maximum residue levels for lincosamide antibiotics in milk. The quantitative IATC assay resulted in a lower detection limit (0.11 μg L?1) by evaluating the colour intensity of the test layers, which was approximately 9 times greater sensitivity than visual detection. During the spike and recovery test, the average recoveries ranged from 76% to 114% at different spiked levels, and the intra-/interday coefficients of variation were in the range 9.6–16.7%. The detection time was shortened to 20 min, whereas the sensitivity was comparable with a traditional ELISA. The IATC assay shows promise for on-site screening of CLIN residues in milk. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
